OBA/Ku86: DNA Binding Specificity and Involvement in Mammalian DNA Replication
نویسندگان
چکیده
منابع مشابه
OBA/Ku86: DNA binding specificity and involvement in mammalian DNA replication.
Ors-binding activity (OBA) was previously semipurified from HeLa cells through its ability to interact specifically with the 186-basepair (bp) minimal replication origin of ors8 and support ors8 replication in vitro. Here, through competition band-shift analyses, using as competitors various subfragments of the 186-bp minimal ori, we identified an internal region of 59 bp that competed for OBA ...
متن کاملCiz1 promotes mammalian DNA replication.
Using a cell-free system that reconstitutes initiation of mammalian DNA replication, we identified a cyclin A-responsive protein, p21(Cip1)-interacting zinc finger protein 1 (Ciz1). In cell-free experiments, Ciz1 protein increases the number of nuclei that initiate DNA replication, and in intact cells GFP-tagged Ciz1 stimulates DNA synthesis, in both a wild-type and a p21(Cip1) null background....
متن کاملHuman placental DNA methyltransferase: DNA substrate and DNA binding specificity.
We have partially purified a DNA methyltransferase from human placenta using a novel substrate for a highly sensitive assay of methylation of hemimethylated DNA. This substrate was prepared by extensive nick translation of bacteriophage XP12 DNA, which normally has virtually all of its cytosine residues replaced by 5-methylcytosine (m5C). Micrococcus luteus DNA was just as good a substrate if i...
متن کاملReplication of Mammalian Dna in Vitro
We have used fiber autoradiography to examine the DNA product made in vitro in a lysed cell system . CHO cells were treated with 0.01% Brij-58 and the lysates were incubated at 30°C in a complete reaction mixture for in vitro DNA synthesis with [ 3H)thymidine triphosphate ([ 3H]TTP) as the radioactive tracer. Fiber autoradiograms prepared from the DNA showed that it was synthesized on tandemly ...
متن کاملDNA-binding specificity of mutant glucocorticoid receptor DNA-binding domains.
Mutation of a small number of amino acids in the DNA-binding domain of the estrogen receptor to the corresponding sequence of the glucocorticoid receptor switches the specificity of the receptor in transactivation assays (Mader, S., Kumar, V., de Verneuil, H., and Chambon, P. (1989) Nature 338, 271-274). We have made the corresponding reciprocal mutations in the context of the glucocorticoid re...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 1999
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.10.3.567